e coli rnap holoenzyme (New England Biolabs)
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Structured Review
New England Biolabs
e coli rnap holoenzyme
E Coli Rnap Holoenzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+rnap+holoenzyme/E%2Ecoli+RNA+Pol%2C+Holoenzyme/bio_rxiv__64898__2026__05__06__723219-162-33-37
Average 97 stars, based on 275 article reviews
E Coli Rnap Holoenzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e+coli+rnap+holoenzyme/E%2Ecoli+RNA+Pol%2C+Holoenzyme/bio_rxiv__64898__2026__05__06__723219-162-33-37
Average 97 stars, based on 275 article reviews
e coli rnap holoenzyme - by Bioz Stars,
2026-10
97/100 stars
Images
1) Product Images from "Blocked transcription-translation complexes are rescued by transcript release followed by trans -translation"
Article Title: Blocked transcription-translation complexes are rescued by transcript release followed by trans -translation
Journal: bioRxiv
doi: 10.64898/2026.05.06.723219
Figure Legend Snippet: FLAG-EGFP and dCas9-sgRNA were expressed in E. coli containing a variant tmRNA with the SsrA-H6D tag (ANDHHHHHHD) or with no tmRNA (Δ ssrA ) and proteins were analyzed by western blotting with ( A ) α-FLAG antibody to visualize all FLAG-EGFP or ( B ) by Penta-His antibody to visualize all SsrA-tagged proteins. Tables above the blots indicate which genes were induced. Strains with no plasmids are included as a negative control (-ctl). The mobilities of FLAG-EGFP bands are indicated. Representative results from three biological repeats are shown. ( C ) FLAG-tagged proteins from the SsrA-H6D strain expressing FLAG-EGFP and dCas9-sgRNA were purified, digested with Asp-N protease, and analyzed by LC-MS/MS. The SsrA-H6D tag was observed after I178 of FLAG-EGFP. The predicted relative locations of the PAM site, the RNA polymerase active site, and the ribosomal P site in the blocked complex are indicated in the diagram, and sequence of full-length FLAG-EGFP in this region is shown. The codon for I178 would be at or near the RNA polymerase active site. The SsrA-H6D tagged peptide from LC-MS/MS is shown below. The first alanine in the AANDHHHHHHD peptide sequence comes from the tRNA-like activity of tmRNA.
Techniques Used: Variant Assay, Western Blot, Negative Control, Expressing, Purification, Liquid Chromatography with Mass Spectroscopy, Sequencing, Activity Assay
Figure Legend Snippet: ( A ) In vitro transcription with or without dCas9-sgRNA. DNA encoding DHFR was incubated with or without dCas9-sgRNA and used as template for in vitro transcription with E. coli RNA polymerase. Transcripts were separated by denaturing urea-PAGE and detected by ethidium bromide staining. ( B ) In vitro transcription-translation and trans -translation reactions with or without dCas9-sgRNA. DNA template encoding full-length DHFR (residues 1-175) was incubated with or without dCas9-sgRNA and transcribed and translated in vitro in the absence or presence of tmRNA-SmpB. Newly synthesized proteins were detected by incorporation of 35 S-methionine and phosphorimaging. Control reactions used truncated DHFR template (residues 1-153) that did not contain a stop codon. Products corresponding to full length DHFR, tagged truncated DHFR, and truncated DHFR are indicated. ( C ) In vitro transcription of DNA template encoding DHFR with a GalR operator site (residues 1-175). ( D ) In vitro translation and trans -translation reactions with or without GalR. Control reactions used truncated DHFR template (residues 1-153 or residues 1-144). Representative images from at least three biological repeats are shown.
Techniques Used: In Vitro, Incubation, Staining, Synthesized, Control
Related Articles
Incubation:Article Title: Directional co-transcriptional folding and pausing create kinetic checkpoints for riboswitch-controlled gene expression Article Snippet: Halted complexes (EC-13) were prepared in transcription buffer (20 mM Tris-HCl, pH 8.0, 20 mM NaCl, 20 mM MgCl 2 , 14 mM 2-mercaptoethanol, 0.1 mM EDTA) containing 25 μM ATP/UTP mix, 50 nM α 32 P-GTP (3000 Ci/mmol), 10 μM ApC dinucleotide primer (Trilink), and 50 nM DNA template. .. Article Title: Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch Article Snippet: Halted complexes (EC-9) were prepared in transcription buffer TB20 (20 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM MgCl 2 , 14 mM 2-mercaptoethanol, 0.1 mM EDTA) containing 25 μM ATP/CTP mix, 50 nM α 32 P-GTP (3000 Ci/mmol), 10 μM ApU dinucleotide primer (Trilink), and 50 nM DNA template. .. Article Title: A leader-repeat hairpin blocks extraneous CRISPR RNA production in diverse CRISPR-Cas13 systems. Article Snippet: For RNase T1 ladders, ~0.2 pmol 32P-5′ end-labeled RNA and 10 μg of yeast RNA (Ambion) was incubated in 1x sequencing buffer (Ambion) for 1 min at 95 °C, transferred on ice and further incubated with 0.1 U RNase T1 (Ambion) for 5 min at 37 °C. .. For that, the DNA templates (50 nM) were preincubated with 100 nM other:Article Title: Co-transcriptional folding orchestrates sequential multi-effector sensing by a glycine tandem riboswitch. Article Snippet: In vitro radioactive transcription assays Halted complexes (EC-9) were prepared in transcription buffer TB20 (20 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM MgCl2, 14 mM 2-mercaptoethanol, 0.1 mM EDTA) containing 25 μM ATP/CTP mix, 50 nM α32P-GTP (3000 Ci/mmol), 10 μM ApU dinucleotide primer (Trilink), and 50 nM DNA template. |
